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highyield t7 azdye647 rna labeling kit  (Jena Bioscience)


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    Jena Bioscience highyield t7 azdye647 rna labeling kit
    Highyield T7 Azdye647 Rna Labeling Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/highyield+t7+rna+labeling+kits/HighYield+T7+AZDye647+RNA+Labeling+Kit/bio_rxiv__64898__2026__04__28__721472-241-10-17
    Average 94 stars, based on 1 article reviews
    highyield t7 azdye647 rna labeling kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: Target gene screening and validation for RNAi-based suppression of the microsporidian parasite Vairimorpha (=Nosema) ceranae in honeybees
    Article Snippet: .. Three technical replicates were performed for each biological replicate, and relative mRNA expression levels were calculated using the 2−△△CT method as described by Livak, Schmittgen [49]. in vitro dsRNA uptake evaluation To assess the uptake of dsRNA by V. ceranae, the dsRNA-swp25 labeled with Cy3 conjugated UTP using HighYield T7 RNA Labeling Kits (Jena Biosciences). .. After in vitro transcription, the strands were annealed and treated with RNase A and DNase I. Unincorporated nucleotides were subsequently removed using Amersham MicroSpin G-25 Columns (Cytiva, Marlborough, MA, USA).

    In Vitro:

    Article Title: Target gene screening and validation for RNAi-based suppression of the microsporidian parasite Vairimorpha (=Nosema) ceranae in honeybees
    Article Snippet: .. Three technical replicates were performed for each biological replicate, and relative mRNA expression levels were calculated using the 2−△△CT method as described by Livak, Schmittgen [49]. in vitro dsRNA uptake evaluation To assess the uptake of dsRNA by V. ceranae, the dsRNA-swp25 labeled with Cy3 conjugated UTP using HighYield T7 RNA Labeling Kits (Jena Biosciences). .. After in vitro transcription, the strands were annealed and treated with RNase A and DNase I. Unincorporated nucleotides were subsequently removed using Amersham MicroSpin G-25 Columns (Cytiva, Marlborough, MA, USA).

    Labeling:

    Article Title: Target gene screening and validation for RNAi-based suppression of the microsporidian parasite Vairimorpha (=Nosema) ceranae in honeybees
    Article Snippet: .. Three technical replicates were performed for each biological replicate, and relative mRNA expression levels were calculated using the 2−△△CT method as described by Livak, Schmittgen [49]. in vitro dsRNA uptake evaluation To assess the uptake of dsRNA by V. ceranae, the dsRNA-swp25 labeled with Cy3 conjugated UTP using HighYield T7 RNA Labeling Kits (Jena Biosciences). .. After in vitro transcription, the strands were annealed and treated with RNase A and DNase I. Unincorporated nucleotides were subsequently removed using Amersham MicroSpin G-25 Columns (Cytiva, Marlborough, MA, USA).



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    (A) Representative immunofluorescence images showing the localization nascent transcripts (EU, green) inside the nucleus (blue) of early GV oocytes. Images show EU levels in negative control (no EU), control (with EU), upon treatment with α-amanitin, and triptolide. Scale bar: 10 µm. (B) Frequency of PSSC with α-amanitin measured by scoring the percentage of eggs with PSSC, similar to levels observed with triptolide . Plots show number of eggs analyzed on top. Statistical significance is measured by Fisher’s exact test, ns = not significant. (C) Representative time-lapse images showing the localization of MajSat RNA in GV and MI oocytes and MII eggs upon microinjection of GV oocytes with <t>UTP-X-Cy3</t> labeled MajSat (top panel) and control (bottom panel) RNA. MajSat RNA localizes to the pericentromeres during metaphase I and metaphase II stages along with foci present in the cytoplasm. Cy3-labeled control RNA localizes only in the cytoplasm and not on chromosomes. MajSat and control RNA (green), chromosomes (H2B-SNAP, blue) are shown. Scale bars: 10 µm. (D) Co-localization of MajSat RNA and SGO1 observed on metaphase I chromosome spreads upon performing RNA FISH with immunofluorescence. Scale bar: 10 µm.
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    (A) Representative immunofluorescence images showing the localization nascent transcripts (EU, green) inside the nucleus (blue) of early GV oocytes. Images show EU levels in negative control (no EU), control (with EU), upon treatment with α-amanitin, and triptolide. Scale bar: 10 µm. (B) Frequency of PSSC with α-amanitin measured by scoring the percentage of eggs with PSSC, similar to levels observed with triptolide . Plots show number of eggs analyzed on top. Statistical significance is measured by Fisher’s exact test, ns = not significant. (C) Representative time-lapse images showing the localization of MajSat RNA in GV and MI oocytes and MII eggs upon microinjection of GV oocytes with <t>UTP-X-Cy3</t> labeled MajSat (top panel) and control (bottom panel) RNA. MajSat RNA localizes to the pericentromeres during metaphase I and metaphase II stages along with foci present in the cytoplasm. Cy3-labeled control RNA localizes only in the cytoplasm and not on chromosomes. MajSat and control RNA (green), chromosomes (H2B-SNAP, blue) are shown. Scale bars: 10 µm. (D) Co-localization of MajSat RNA and SGO1 observed on metaphase I chromosome spreads upon performing RNA FISH with immunofluorescence. Scale bar: 10 µm.
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    Jena Bioscience highyield t7 rna labelling kit
    (A) Representative immunofluorescence images showing the localization nascent transcripts (EU, green) inside the nucleus (blue) of early GV oocytes. Images show EU levels in negative control (no EU), control (with EU), upon treatment with α-amanitin, and triptolide. Scale bar: 10 µm. (B) Frequency of PSSC with α-amanitin measured by scoring the percentage of eggs with PSSC, similar to levels observed with triptolide . Plots show number of eggs analyzed on top. Statistical significance is measured by Fisher’s exact test, ns = not significant. (C) Representative time-lapse images showing the localization of MajSat RNA in GV and MI oocytes and MII eggs upon microinjection of GV oocytes with <t>UTP-X-Cy3</t> labeled MajSat (top panel) and control (bottom panel) RNA. MajSat RNA localizes to the pericentromeres during metaphase I and metaphase II stages along with foci present in the cytoplasm. Cy3-labeled control RNA localizes only in the cytoplasm and not on chromosomes. MajSat and control RNA (green), chromosomes (H2B-SNAP, blue) are shown. Scale bars: 10 µm. (D) Co-localization of MajSat RNA and SGO1 observed on metaphase I chromosome spreads upon performing RNA FISH with immunofluorescence. Scale bar: 10 µm.
    Highyield T7 Rna Labelling Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Representative immunofluorescence images showing the localization nascent transcripts (EU, green) inside the nucleus (blue) of early GV oocytes. Images show EU levels in negative control (no EU), control (with EU), upon treatment with α-amanitin, and triptolide. Scale bar: 10 µm. (B) Frequency of PSSC with α-amanitin measured by scoring the percentage of eggs with PSSC, similar to levels observed with triptolide . Plots show number of eggs analyzed on top. Statistical significance is measured by Fisher’s exact test, ns = not significant. (C) Representative time-lapse images showing the localization of MajSat RNA in GV and MI oocytes and MII eggs upon microinjection of GV oocytes with UTP-X-Cy3 labeled MajSat (top panel) and control (bottom panel) RNA. MajSat RNA localizes to the pericentromeres during metaphase I and metaphase II stages along with foci present in the cytoplasm. Cy3-labeled control RNA localizes only in the cytoplasm and not on chromosomes. MajSat and control RNA (green), chromosomes (H2B-SNAP, blue) are shown. Scale bars: 10 µm. (D) Co-localization of MajSat RNA and SGO1 observed on metaphase I chromosome spreads upon performing RNA FISH with immunofluorescence. Scale bar: 10 µm.

    Journal: bioRxiv

    Article Title: Restoring Shugoshin 1 reduces chromosome errors in human eggs

    doi: 10.64898/2026.01.08.698387

    Figure Lengend Snippet: (A) Representative immunofluorescence images showing the localization nascent transcripts (EU, green) inside the nucleus (blue) of early GV oocytes. Images show EU levels in negative control (no EU), control (with EU), upon treatment with α-amanitin, and triptolide. Scale bar: 10 µm. (B) Frequency of PSSC with α-amanitin measured by scoring the percentage of eggs with PSSC, similar to levels observed with triptolide . Plots show number of eggs analyzed on top. Statistical significance is measured by Fisher’s exact test, ns = not significant. (C) Representative time-lapse images showing the localization of MajSat RNA in GV and MI oocytes and MII eggs upon microinjection of GV oocytes with UTP-X-Cy3 labeled MajSat (top panel) and control (bottom panel) RNA. MajSat RNA localizes to the pericentromeres during metaphase I and metaphase II stages along with foci present in the cytoplasm. Cy3-labeled control RNA localizes only in the cytoplasm and not on chromosomes. MajSat and control RNA (green), chromosomes (H2B-SNAP, blue) are shown. Scale bars: 10 µm. (D) Co-localization of MajSat RNA and SGO1 observed on metaphase I chromosome spreads upon performing RNA FISH with immunofluorescence. Scale bar: 10 µm.

    Article Snippet: The HighYield T7 Cy3 RNA Labelling Kit (Jena Bioscience, #RNT-101-CY3) was used to label amplified sequences with Cy3-labelled UTP (UTP-X-Cy3, 35%), followed by DNA removal using TurboTM DNAse (ThermoFisher).

    Techniques: Immunofluorescence, Negative Control, Control, Microinjection, Labeling